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Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed Macaque Populations in Bali Island, Indonesia

Drh. I Gede Soma M.Kes. , I GEDE SOMA (2014) Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed Macaque Populations in Bali Island, Indonesia. JURNAL ILMU DAN KESEHATAN HEWAN, 2. ISSN 2302-6057

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Abstract

ABSTRAK Monyet ekor panjang tersebar di seluruh wilayah di Pulau Bali, tetapi, kini keberadaannya telah terpisah-pisah menjadi populasi lokal yang lebih kecil. Walupun demikian, laporan yang melukiskan modus fragmentasi dan struktur genetik populasi belum banyak ditemukan. Penelitian ini bertujuan untuk mengeksplorasi polimorfisme lokus mikrosatellite kromosom Y pada populasi monyet ekor panjang di Pulau Bali dengan menggunanakan tiga primer mikrosatelit manusia, yaitu DYS390, DYS391, dan DYS393. Sejumlah 99 sampel darah dikoleksi dari monyet ekor panjang jantan yang berasal dari 8 populasi (23 dari Pulaki, 11 dari Bedugul, 13 dari Mekori, 8 dari Sangeh, 6 dari Uluwatu, 11 dari Alas Kedaton, 11 dari Ubud, dan 16 dari Bukit Gumang). DNA total diekstraksi dengan QIAamp® Blood Mini Kit. Polimorfisme lokus mikrosatelit dideteksi menggunkan teknik PCR, dan variasi alel dipisahkan melalui elektroforesis pada 7% gel poliakrilamid. Hasil penelitian menunjukkan bahwa lokus DYS390 dan DYS393 adalah monomorfik (masing-masing memiliki satu alel). Lokus DYS391 mempunyai dua alel, karena itu, lokus ini adalah polimorfik. Dari hasil penelitian dapat disarankan bahwa lokus DYS391 dapat digunakan untuk studi variasi genetik populasi monyet ekor panjang jantan di Pulau Bali. Kata kunci: monyet ekor panjang, polimorfisme, lokus mikrosatelit, kromosom Y, Pulau Bali ABSTRACT The long tailed macaques inhabit throughout Bali Island, however, recently they have been fragmented into many smaller local populations. In spite of that, there are just few reports that describe the mode of fragmentation and the genetic structure of the population. This research aimed to explore the polymorphism of microsatellite loci on Y chromosome in long tailed macaque populations in Bali Island using three human microsatellite primers, namely DYS390, DYS391, and DYS393. A total of 99 blood samples were collected from male long tailed macaques originated from 8 populations (23 from Pulaki, 11 from Bedugul, 13 from Mekori, 8 from Sangeh, 6 from Uluwatu, 11 from Alas Kedaton, 11 from Ubud, and 16 from Bukit Gumang). Total DNA was extracted using QIAamp® Blood Mini Kit. The polymorphism of the microsatellite loci was detected using PCR technique, and allelic variations were separated through 7% poly acrylamide gel electrophoresis. The result of the research showed that DYS390 and 1,4 Wandia et al. Jurnal Ilmu dan Kesehatan Hewan, Agustus 2014 DYS393 loci were monomorphic (each had only one allele). The DYS391 locus had two alleles, therefore, this locus was polymorphic. It could be suggested that DYS391 locus could be used to study genetic variation in male long tailed macaque population in Bali Island. Key words: long tailed macaques, polymorphism, microsatellite locus, Y chromosome, Bali Island

Item Type: Article
Uncontrolled Keywords: -
Subjects: L Education > L Education (General)
Divisions: Faculty of Law, Arts and Social Sciences > School of Education
Depositing User: Mr. Repository Admin
Date Deposited: 07 Jun 2016 21:58
Last Modified: 21 Jun 2016 05:58
URI: http://erepo.unud.ac.id/id/eprint/171

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