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Identifikasi Mutasi Gen rpob Isolat MDR Mycobacterium tuberculosis di Bali dengan Metode Nested PCR

Dr.I NENGAH WIRAJANA,S.Si.,M.Si, I Nengah Wirajana (2014) Identifikasi Mutasi Gen rpob Isolat MDR Mycobacterium tuberculosis di Bali dengan Metode Nested PCR. Jurnal Farmasi Indonesia, 7. ISSN Print ISSN 1412-1107 dan e-ISSN 2355-696X

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Abstract

Tuberculosis is one of the infectious diseases that is important to be treated in Millenium Development Goals (MDGs) programme, mainly due to the increasing of Multidrug resistant tuberculosis (MDR-TB) cases. According to WHO, MDR-TB was defined as tuberculosis caused by strains of Mycobacterium tuberculosis that are resistant to at least isoniazid and rifampicin. Several studies found that nearly 90% of rifampin-resistant isolates, were also resistant to isoniazid. Therefore, resistance to rifampicin is a surrogate marker of MDR. Resistance to rifampicin is mediated by mutation in a region of 81 bp (RRDR: Rifampicin Resistance Determinant Region) of the rpoB gene. The aim of this research mainly was to explore mutation of MDR-TB’ rpoB gene of tuberculosis patient in Bali. All MDR-TB isolates used for this research were the collection of Clinical Microbiology Laboratory Of Sanglah Hospital, Denpasar. Amplification were conducted by Nested Polymerase Chain Reaction methods using two pair of inner and outer primers. The result of this study showed that except at RRDR, five of six isolates had one similar mutation at codon 418 from glutamic acid to aspartic acid. Therefore, it could be concluded that there has been a missense mutation in all isolates.

Item Type: Article
Uncontrolled Keywords: -
Subjects: L Education > L Education (General)
Divisions: Faculty of Law, Arts and Social Sciences > School of Education
Depositing User: Mr. Repository Admin
Date Deposited: 07 Jun 2016 21:58
Last Modified: 21 Jun 2016 05:58
URI: http://erepo.unud.ac.id/id/eprint/3511

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