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Supplementation of Cantharanthus Roseus leaf Extract as Anti-Inflammatory Substance to Hypercholesterolemic Wistar Derived-Rats

Dr. Drs. Anak Agung Ngurah Gunawan,MT, ANAK AGUNG NGURAH GUNAWAN (2014) Supplementation of Cantharanthus Roseus leaf Extract as Anti-Inflammatory Substance to Hypercholesterolemic Wistar Derived-Rats. Pure And Applied Chemical Sciences, 3. ISSN 1314-7633

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Abstract

The present study on supplementation of Cantharanthus roseus leaf extract (CRLE) to rats suffer from hypercholesterolemia was aimed to determine whether it has property as an anti-inflammatory substance assessed from concentrations of tumor necrosis factor-α (TNF- α) and Interleukin-6 (IL-6) in the blood serum. A randomized pre-test and post-test design was employed, using 4 weeks old Wistar derived-rats at body weight ranging from 70 – 72 gram. The rats were initially fed with normal rat pellets for 1 month followed by high fat ration for 6 weeks in order to induce hypercholesterolemia condition. Then they were divided into 5 groups according to various treatments applied as follows. Group 1: control – supplemented with 0% CRLE; Group 2: 10% CRLE; Group 3: 15% CRLE; Group 4: 20% CRLE; and Group 5: 25% CRLE. CRLE supplementation was last for 1 month. TNF- αconcentration was determined using Sandwich Enzyme (ELISA) kit spectrophotometer (at π =500 nm) andconcentration of IL-6 was determined using Quintikine HS immunoassay kit. In general, results of the current study showed that CRLE may reduce inflammation based on assessment of concentrations of TNF- α and IL-6 in the serum. Following supplementation of 20% CRLE, TNF- α concentration decreased from 28.62 pg/ml to 15.56 pg/ml while IL-6 decreased from 134.64 g/ml to 113.87 pg/ml. In Indonesia, Cantharanthus roseus grown as ornament plant and contain tannin, tripernoids, and alkaloids. The component that acts an anti-inflammation is flavanoids.

Item Type: Article
Uncontrolled Keywords: Leaf of Cantharanthus roseus, tumor necrosis facto
Subjects: L Education > L Education (General)
Divisions: Faculty of Law, Arts and Social Sciences > School of Education
Depositing User: Mr. Repository Admin
Date Deposited: 07 Jun 2016 21:58
Last Modified: 21 Jun 2016 05:58
URI: http://erepo.unud.ac.id/id/eprint/5431

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