UNUD Open Repository

UNUD Open Repository provides access and discovery to the University of Udayana publications and digital collections. It contains digitized and digital version of theses, dissertations, research reports, and articles produced by academic communities in this university.

Supplementation of Cantharanthus Roseus leaf Extract as Anti-Inflammatory Substance to Hypercholesterolemic Wistar Derived-Rats

Dr. Drs. Anak Agung Ngurah Gunawan,MT, ANAK AGUNG NGURAH GUNAWAN (2014) Supplementation of Cantharanthus Roseus leaf Extract as Anti-Inflammatory Substance to Hypercholesterolemic Wistar Derived-Rats. Pure And Applied Chemical Sciences, 3. ISSN 1314-7633

[img] Archive

Download (525kB)


The present study on supplementation of Cantharanthus roseus leaf extract (CRLE) to rats suffer from hypercholesterolemia was aimed to determine whether it has property as an anti-inflammatory substance assessed from concentrations of tumor necrosis factor-α (TNF- α) and Interleukin-6 (IL-6) in the blood serum. A randomized pre-test and post-test design was employed, using 4 weeks old Wistar derived-rats at body weight ranging from 70 – 72 gram. The rats were initially fed with normal rat pellets for 1 month followed by high fat ration for 6 weeks in order to induce hypercholesterolemia condition. Then they were divided into 5 groups according to various treatments applied as follows. Group 1: control – supplemented with 0% CRLE; Group 2: 10% CRLE; Group 3: 15% CRLE; Group 4: 20% CRLE; and Group 5: 25% CRLE. CRLE supplementation was last for 1 month. TNF- αconcentration was determined using Sandwich Enzyme (ELISA) kit spectrophotometer (at π =500 nm) andconcentration of IL-6 was determined using Quintikine HS immunoassay kit. In general, results of the current study showed that CRLE may reduce inflammation based on assessment of concentrations of TNF- α and IL-6 in the serum. Following supplementation of 20% CRLE, TNF- α concentration decreased from 28.62 pg/ml to 15.56 pg/ml while IL-6 decreased from 134.64 g/ml to 113.87 pg/ml. In Indonesia, Cantharanthus roseus grown as ornament plant and contain tannin, tripernoids, and alkaloids. The component that acts an anti-inflammation is flavanoids.

Item Type: Article
Uncontrolled Keywords: Leaf of Cantharanthus roseus, tumor necrosis facto
Subjects: L Education > L Education (General)
Divisions: Faculty of Law, Arts and Social Sciences > School of Education
Depositing User: Mr. Repository Admin
Date Deposited: 07 Jun 2016 21:58
Last Modified: 21 Jun 2016 05:58
URI: http://erepo.unud.ac.id/id/eprint/5431

Actions (login required)

View Item View Item