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The Trivhoderma asperillum TKD filtrate potency in reducing contaminants of aflatoxin B1 by apregillus flavus FNCC6109 on concentrate feed

Dr. Drs. Ida Bagus Gede Darmayasa,M.Si., IDA BAGUS GEDE DARMAYASA (2014) The Trivhoderma asperillum TKD filtrate potency in reducing contaminants of aflatoxin B1 by apregillus flavus FNCC6109 on concentrate feed. International Journal of Pure & Applied Bioscience, 2(6). ISSN 2320-7051

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Abstract

Aspergillus flavus is a fungus that often contaminates concentrate feed. This fungus is able to produce aflatoxin B1, which is harmful to livestock and humans. The aim of this study is to determine the potential of the filtrate of Trichoderma asperillum TKD in reducing aflatoxin B1 produced by Aspergillus flavus FNCC 6109. The test is done by formulating concentrate feed with filtrate concentrations of 20 %; 40 % and 60 %. The level of aflatoxin B1 is determined by ELISA technique (Enzyme Linked Immuno Sorbent Assay). The characterization of secondary metabolites in the filtrate is analyzed by using GCMS. The results showed that 60 % of filtrate concentration was able to reduce aflatoxin B1 by 47.84 % which is significantly different (P?0,05) to other treatments. The secondary metabolites contained in T. asperillum TKD filtrate are Butane, 1,1 - oxybis- (CAS) n - Butyl ether; Butane, 1,1 - dibutoxy - ; Pentansaeure, 2,24,4 - Tetramethyl; 1 - Tetradecene; Phenol, 2,4 - bis (1,1 - dimethylethyl- (CAS) 2,4; 1- Hexadecane (CAS) Cetene; 1 - Octadecene (CAS) alpha - Octadecene ; hexadecanoic acid , methyl ester (CAS) Methyl, n - Tetracosano - 1 , 4 - Heptanone (CAS) GBL; 1 - Dodecanol (CAS) n - Dodecanol and Behenic alcohol. Keywords: aflatoxin B1, filtrate, T. asperillum TKD, concentrate.

Item Type: Article
Uncontrolled Keywords: -
Subjects: L Education > L Education (General)
Divisions: Faculty of Law, Arts and Social Sciences > School of Education
Depositing User: Mr. Repository Admin
Date Deposited: 07 Jun 2016 21:58
Last Modified: 21 Jun 2016 05:58
URI: http://erepo.unud.ac.id/id/eprint/711

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