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HUBUNGAN POSITIF EKSPRESI CYCLOOXYGENASE-2 DENGAN MICROVESSEL DENSITY PADAUNDIFFERENTIATEDCARCINOMANASOPHARYNXDI RSUP SANGLAH DENPASAR

Dr. dr. I Gusti Ayu Sri Mahendra Dewi, Sp.PA(K), I GUSTI AYU SRI MAHENDRA DEWI (2017) HUBUNGAN POSITIF EKSPRESI CYCLOOXYGENASE-2 DENGAN MICROVESSEL DENSITY PADAUNDIFFERENTIATEDCARCINOMANASOPHARYNXDI RSUP SANGLAH DENPASAR. Majalah Patologi Indonesia, 26 No.. ISSN 0215-7282

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Abstract

ABSTRAK HUBUNGAN POSITIF EKSPRESI CYCLOOXYGENASE-2 DENGAN MICROVESSEL DENSITY PADA UNDIFFERENTIATED CARCINOMA NASOPHARYNX DI RSUP SANGLAH DENPASAR Tumor memerlukan pembentukan pembuluh darah baru atau angiogenesis untuk dapat tumbuh dan bermetastasis. Angiogenesis dapat dinilai dengan menghitung microvessel density (MVD). Salah satu cara untuk menentukan microvessel adalah dengan pengecatan imunohistokimia CD31. Cyclooxygenase-2 (COX-2) adalah faktor potensial penting pada angiogenesis. Tujuan penelitian ini adalah mengetahui hubungan ekspresi COX-2 dengan MVD pada undifferentiated carcinoma nasopharynx di RSUP Sanglah Denpasar. Penelitian ini menggunakan metode analitik potong lintang. Sampel penelitian adalah sediaan blok parafin penderita undifferentiated carcinoma nasopharynx yang diperiksa secara histopatologi pada Bagian/SMF Patologi Anatomi FK UNUD/RSUP Sanglah Denpasar dan Laboratorium Patologi Anatomi FK Universitas Gadjah Mada/RSUP dr.Sardjito, Yogyakarta dari 1 Januari 2014 sampai dengan 31 Agustus 2014. Diagnosis ulang sediaan histopatologi dilakukan dengan pengecatan rutin H&E untuk mendapatkan sampel yang memenuhi kriteria inklusi dan eksklusi sehingga tercapai jumlah 31 sampel.Selanjutnya dilakukan pulasan imunohistokimia COX-2 dan CD 31 untuk menentukan microvessel pada seluruh sampel.Kemudian hasil dianalisis dengan uji Pearson. Ekspresi COX-2 positif ditemukan pada 24 (77,42%) subyek dan dijumpai negatif pada 7 (22,59%). Ditemukan 22 (70,97%) kasus undifferentiated carcinoma nasopharynx dengan MVD tinggi dan 9 (29,03%) dengan MVD rendah. Ditemukan adanya korelasi positif ekspresi COX-2 dengan MVD (r = 0,868; p = 0,001). Pada penelitian ini, ditemukan adanya hubungan positif antara ekspresi COX-2 dan MVD pada undifferentiated carcinoma nasopharynx. Kata kunci :undifferentiated carcinoma nasopharynx, Cyclooxygenase-2, Microvessel density. ABSTRACT POSITIVE CORRELATION BETWEEN EXPRESSION CYCLOOXYGENASE-2 AND MICROVESSEL DENSITY IN UNDIFFERENTIATED CARCINOMA NASOPHARYNX AT RSUP SANGLAH DENPASAR Tumors require new blood vessel formation or angiogenesis in order to grow and metastasize. Angiogenesis can be assessed by counting the microvessel density (MVD). One way to determine microvessel is the CD31 immunohistochemical staining. Cyclooxygenase-2 (COX-2) is a potentially important factor in angiogenesis.The aim of this study was to prove the correlation between expression COX-2 and MVD in undifferentiated carcinoma nasophrynx at RSUP Sanglah Denpasar. This study used cross-sectional analytic method . The sample were paraffin block preparation of patients with undifferentiated carcinoma of the nasopharynx were examined by histopathology in Pathology Anatomy Departement, Medical Faculty Udayana University/RSUP Sanglah Denpasar and Laboratory of Anatomical Pathology, Faculty of Medicine, University of Gadjah Mada/DR.Sardjito Hospital, Yogyakarta from January 1, 2014 to August 31, 2014. Histopathological diagnosis performed on preparations with routine H & E staining to obtain samples that met the inclusion and exclusion criteria in order to reach the number of 31 samples. Subsequently immunohistochemical staining was performed for COX-2 and CD 31 to determined microvessel on the entire sample. Then the results were analyzed by Pearson test. COX-2 positive expression were found in 24 (77.42%) subjects were found negative in 7 (22.59%). Twenty two cases (70.97%) cases of undifferentiated carcinoma of the nasopharynx with high MVD and 9 (29.03%) with low MVD. There was positive correlation expression of COX - 2 with MVD ( r = 0.868 ; p = 0.001 ) . In this study , found a positive correlation between the expression of COX- 2 and MVD in undifferentiated carcinoma nasopharynx Keywords: undifferentiated carcinoma nasopharynx, cylooxygenase-2, microvessel density

Item Type: Article
Uncontrolled Keywords: Keywords: undifferentiated carcinoma nasopharynx, cylooxygenase-2, microvessel density
Subjects: L Education > L Education (General)
Divisions: Faculty of Law, Arts and Social Sciences > School of Education
Depositing User: Mr. Repository Admin
Date Deposited: 07 Jun 2016 21:58
Last Modified: 21 Jun 2016 05:58
URI: http://erepo.unud.ac.id/id/eprint/7861

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