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1.1. Variability of Reverse Transcriptase gene and Hepatitis B Virus Genotyping among HBsAg-positive Blood Donors in Denpasar, Bali, Indonesia

Dr. dr. Anak Agung Wiradewi Lestari, S.Ked, Sp.PK, Anak Agung Wiradewi Lestari (2014) 1.1. Variability of Reverse Transcriptase gene and Hepatitis B Virus Genotyping among HBsAg-positive Blood Donors in Denpasar, Bali, Indonesia. Proceeding The 1st International Conference on Science and Technology Applications in Climate Change, -. ISSN ISBN 978-983-44827-4-9

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Abstract

Hepatitis B Virus (HBV) resistance to anti-HBV drugs has become a major problem in treating HBV infections. Reverse transcriptase (RT), the viral protein that is important for viral replication, is target of anti-HBV drugs. This study was performed in order to analyse genetic variability among RT gene and to determine HBV genotype among healthy carrier blood donor individuals in Denpasar, Bali, Indonesia. Serum samples were collected from 53 blood donors who were HBsAg-positive. Mutation of RT gene, and genotype of HBV were determined using PCR and sequencing analysis. About 32 (60.4%) of serum samples were PCR- positive, and then sequenced for investigating RT mutation and HBV genotype analysis. None of primary mutation in domain A, B, C, D, and E in RT gene was observed. Furthermore, type B (B3) was the predominant genotype of HBV among this group (27 out of 32 positive PCR sera (84.4%)). There were 4 (12.5%) of isolates was type D, and only 1 isolates (3.1%) was type C. This study provides information for further study related to HBV molecular characterization in Bali. Further investigation among chronic Hepatitis B virus patients under therapy is important to be conducted.

Item Type: Article
Uncontrolled Keywords: Hepatitis B virus, Reverse Transcriptase, Resistance, Genotype, Blood Donors
Subjects: L Education > L Education (General)
Divisions: Faculty of Law, Arts and Social Sciences > School of Education
Depositing User: Mr. Repository Admin
Date Deposited: 07 Jun 2016 21:58
Last Modified: 21 Jun 2016 05:58
URI: http://erepo.unud.ac.id/id/eprint/7873

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