Isolation and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of active compound from Jujube tree (Zizyphus mauritiana Auct. non Lamk.)

apt. PUTU OKA SAMIRANA, S.Farm., M.Sc., PUTU OKA SAMIRANA (2017) Isolation and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of active compound from Jujube tree (Zizyphus mauritiana Auct. non Lamk.). International Journal of Food Properties, 20 (S2). ISSN 1094-2912

[img] Archive
6edf6549336523e2bc76ffa768f885a4.pdf - Published Version

Download (4MB)

Abstract

Increased reactive oxygen species causes cells or tissue damages and is associated
with some degenerative diseases, such as coronary heart diseases and
cancer. This research is intended to isolate and to identify the antiradical
compounds from the bark of Jujube tree (Zizyphus mauritiana Auct. non
Lamk.). The methanol extract of the bark was successively fractionated with
n-hexane and ethyl acetate to give n-hexane fraction, ethyl acetate fraction, and
methanol extract residue, respectively. The extract and fractions were examined
for its radical scavenging activity using 2,2’-diphenyl-1-picrylhydrazil radical. The
ethyl acetate fraction, having the most active 2,2’-diphenyl-1-picrylhydrazil radical
scavenging, was further repeatedly separated using vacuum liquid chromatography,
and the purification was carried out using preparative thin layer
chromatography (TLC) in order to obtain an active isolate. The purity of the
isolate was evaluated using thin layer chromatography method and melting
point measurement. The structure of the isolate was identified chemically using
various spray reagent and ultraviolet-visible, infrared, infrared, Liquid
Chromatography-Mass Spectrometry (LC-MS) and Proton Magnetic Resonance
(1H-NMR) spectroscopic methods. The extract, fraction, and sub-fractions were
determined for its total contents of phenolic and flavonoid. The results showed
that the methanol extract residue contained the highest total phenolic (36.29%
wt/wt gallic acid equivalent), while fraction E4 of the ethyl acetate extract
contained the highest total flavonoids (21.31% wt/wt quercetin equivalent).
The active isolate showed to have 2,2’-diphenyl-1-picrylhydrazil radical scavenging
activity with IC50 of 7.58 ?/mL and is identified as trans-p-coumaroyl
triterpene. Detail structure of the triterpene needs to be identified further.

Item Type: Article
Subjects: L Education > L Education (General)
Divisions: Faculty of Law, Arts and Social Sciences > School of Education
Depositing User: USDI USDI Universitas Udayana
Date Deposited: 20 Jul 2020 12:24
Last Modified: 20 Jul 2020 12:24
URI: https://erepo.unud.ac.id/id/eprint/19593

Actions (login required)

View Item View Item