Bioactivity of Fungi Trichoderma reesei Associated with Sponges Stylissa flabelliformis Collected from National Park West Bali, Indonesia

apt. PUTU OKA SAMIRANA, S.Farm., M.Sc., PUTU OKA SAMIRANA (2017) Bioactivity of Fungi Trichoderma reesei Associated with Sponges Stylissa flabelliformis Collected from National Park West Bali, Indonesia. Journal of Biological Sciences, 17 (8). ISSN 1727-3048

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Abstract

Objective: In this study, antimicrobial and cytotoxic properties of ethyl acetate extract of fermentedT richoderma reesei (T. reesei )fungi
associated with the sponge Stylissa flabelliformis (S. flabelliformis ) was tested. Materials and Methods: Fermentation of T. reesei was
carried out in the saboroud dextrosa saline broth. The fermented filtrate was extracted using ethyl acetate. The ethyl acetate extract was
tested for its antimicrobial and cytotoxic activity. Thin-layer chromatography and gas chromatography mass spectrometry were performed
to determine the metabolite classes of compounds responsible for antimicrobial and cytotoxic activity. Results: Ethyl acetate extract
ofT . reesei was active against the bacteriaS taphylococcus aureus ATCC 29213,E schericia coli ATCC 25922 andC andida albicans ATCC
10231. The ethyl acetate extract was also active against cancer cells T47D and Raji cells, with IC50 values of 270 and 470 ?g mLG1,
respectively. Conclusion: An ethyl acetate extract ofT richoderma reesei fermentations showed antimicrobial and cytotoxic activity. The
compounds contained in the extract of T. reesei are terpenoids or phenyl propane derivatives.

Item Type:	Article
Subjects:	L Education > L Education (General)
Divisions:	Faculty of Law, Arts and Social Sciences > School of Education
Depositing User:	USDI USDI Universitas Udayana
Date Deposited:	20 Jul 2020 12:24
Last Modified:	20 Jul 2020 12:24
URI:	https://erepo.unud.ac.id/id/eprint/19706

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